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LearnPAB14105
antibody from Abnova Corporation
Targeting: L3MBTL1
dJ138B7.3, DKFZp586P1522, KIAA0681, L3MBTL, ZC2HC3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- PAB14105 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14105, RRID:AB_10555320
- Product name
- L3MBTL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against L3MBTL.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The human L(3)MBT polycomb group protein is a transcriptional repressor and interacts physically and functionally with TEL (ETV6).
Boccuni P, MacGrogan D, Scandura JM, Nimer SD
The Journal of biological chemistry 2003 Apr 25;278(17):15412-20
The Journal of biological chemistry 2003 Apr 25;278(17):15412-20
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on HeLa nuclear extract (HeLa NE) using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. The antibody recognizes endogenous L3MBTL1 in HeLa nuclear extract (HeLa NE). The location of L3MBTL1 is indicated with an arrow.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000.The antibody recognizes the immunogen (GST-L1N) in the ng range (Lanes 1-3).L3MBTL polyclonal antibody (Cat # PAB14105) also detects ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) (Lane 5).L3MBTL polyclonal antibody (Cat # PAB14105) also recognizes a protein of the correct size (approx. 100 kDa) (end. L3MBTL1 : endogenous L3MBTL1) in nuclear extract from HeLa (Lane 8) and HEK293 cells (Lane 7), but not in ML-2 cells (Lane 6).A negative control (MOCK) is shown (Lane 4).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on nuclear extracts containing ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) using L3MBTL polyclonal antibody (Cat # PAB14105) and an IgG control antibody (IgG Control).The presence of L3MBTL1-F in the input (In ; Lane 1), in the precipitated fraction (P) and in the supernatant fraction (SN) was tested by Western Blot using anti-FLAG antibody.L3MBTL1-F was efficiently precipitated by L3MBTL polyclonal antibody (Cat # PAB14105) (Lane 3), since there was no L3MBTL1-F present in the supernatant (Lane 5).L3MBTL1-F was not precipitated by the control antibody (IgG Control) (Lane 2) and the L3MBTL1 was still present in the supernatant (Lane 4).
- Validation comment
- Immunoprecipitation
