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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-24624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2X2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-24624 is expected to cross react with mouse (100% conserved) and guinea pig (100% conserved) due to sequence homology.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Vagal afferent fibers contribute to the anti-inflammatory reactions by vagus nerve stimulation in concanavalin A model of hepatitis in rats.
Jo BG, Kim SH, Namgung U
Molecular medicine (Cambridge, Mass.) 2020 Dec 3;26(1):119
Molecular medicine (Cambridge, Mass.) 2020 Dec 3;26(1):119
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of rat brain stem using (Product # PA1-24624) P2X2 Polyclonal Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Immunofluorescence identification of degenerating NG neurons in CAP-treated animals. a Colocalization of DiI-labeled neurons with NF-200-immunostained neurons in the NG. Seven days after DiI injection into the hepatic vagal branch in rats, NG was dissected and subjected to immunofluorescence staining for NF-200. b - e Immunofluorescence staining of TRPV1 and P2X2 proteins in the NG. Three days after CAP injection with a total of 100 mg/kg (CAP-L) or 200 mg/kg (CAP-H), NG sections were prepared for immunofluorescence staining for TRPV1 and P2X2 receptor proteins ( b ) or for cleaved form of caspase 3 with Hoechst 33258 nuclear staining ( d ). The number of neurons labeled with TRPV1 and caspase 3 proteins were quantified and plotted in c and e , respectively. **p < 0.01, ***p < 0.001 (One way ANOVA with Tukey post hoc, n = 4). Scale bars in a , b , and d are 100 mum. f Eye wiping test in rats. Eye wipe frequency during 5 min period was measured and compared between CAP- and VEH-injected animals. ***p < 0.001, t = 10.74 (unpaired t-test, n = 4)
