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- Antigen structure
- References [6]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 22587-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- WBP2NL antibody
- Antibody type
- Polyclonal
- Description
- WBP2NL antibody (Cat. #22587-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A homozygous nonsense mutation of PLCZ1 cause male infertility with oocyte activation deficiency.
Oligoasthenoteratozoospermic (OAT) men display altered phospholipase C ζ (PLCζ) localization and a lower percentage of sperm cells expressing PLCζ and post-acrosomal sheath WW domain-binding protein (PAWP).
Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.
PLCζ is the physiological trigger of the Ca2+ oscillations that induce embryogenesis in mammals but conception can occur in its absence.
Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Wang F, Zhang J, Kong S, Li C, Zhang Z, He X, Wu H, Tang D, Zha X, Tan Q, Duan Z, Cao Y, Zhu F
Journal of assisted reproduction and genetics 2020 Apr;37(4):821-828
Journal of assisted reproduction and genetics 2020 Apr;37(4):821-828
Oligoasthenoteratozoospermic (OAT) men display altered phospholipase C ζ (PLCζ) localization and a lower percentage of sperm cells expressing PLCζ and post-acrosomal sheath WW domain-binding protein (PAWP).
Azad N, Nazarian H, Ghaffari Novin M, Masteri Farahani R, Piryaei A, Heidari MH, Abdollahpour Alitappeh M
Bosnian journal of basic medical sciences 2018 May 20;18(2):178-184
Bosnian journal of basic medical sciences 2018 May 20;18(2):178-184
Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.
Kashir J, Buntwal L, Nomikos M, Calver BL, Stamatiadis P, Ashley P, Vassilakopoulou V, Sanders D, Knaggs P, Livaniou E, Bunkheila A, Swann K, Lai FA
Molecular human reproduction 2017 Jan;23(1):54-67
Molecular human reproduction 2017 Jan;23(1):54-67
PLCζ is the physiological trigger of the Ca2+ oscillations that induce embryogenesis in mammals but conception can occur in its absence.
Hachem A, Godwin J, Ruas M, Lee HC, Ferrer Buitrago M, Ardestani G, Bassett A, Fox S, Navarrete F, de Sutter P, Heindryckx B, Fissore R, Parrington J
Development (Cambridge, England) 2017 Aug 15;144(16):2914-2924
Development (Cambridge, England) 2017 Aug 15;144(16):2914-2924
Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Escoffier J, Lee HC, Yassine S, Zouari R, Martinez G, Karaouzène T, Coutton C, Kherraf ZE, Halouani L, Triki C, Nef S, Thierry-Mieg N, Savinov SN, Fissore R, Ray PF, Arnoult C
Human molecular genetics 2016 Mar 1;25(5):878-91
Human molecular genetics 2016 Mar 1;25(5):878-91
Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Nomikos M, Sanders JR, Kashir J, Sanusi R, Buntwal L, Love D, Ashley P, Sanders D, Knaggs P, Bunkheila A, Swann K, Lai FA
Molecular human reproduction 2015 Sep;21(9):702-10
Molecular human reproduction 2015 Sep;21(9):702-10
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse testis tissue were subjected to SDS PAGE followed by western blot with 22587-1-AP( WBP2NL Antibody) at dilution of 1:600
- Other comments
- WB
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 22587-1-AP( WBP2NL Antibody) at dilution of 1:50 (under 10x lens)
- Other comments
- IHC
