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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 502928 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#502928, RRID:AB_2561315
- Product name
- Alexa Fluor® 700 anti-human TNF-a
- Antibody type
- Monoclonal
- Antigen
- E. coli-expressed, recombinant human TNF-α
- Reactivity
- Human, Porcine
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Antibody clone number
- MAb11
- Vial size
- 100 tests
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed, permeabilized and stained with TNF-α (clone MAb11) Alexa Fluor® 700 (top). The bottom dot plot shows the staining can by blocked by pre-incubation with unlabeled purified MAb11 antibody.
- Conjugate
- Near infrared dye
