502939

antibody from BioLegend

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

502939

Provider

BioLegend

Proper citation

BioLegend Cat#502939, RRID:AB_2562740

Product name

Brilliant Violet 711™ anti-human TNF-a

Antibody type

Monoclonal

Antigen

E. coli-expressed, recombinant human TNF-α

Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4⁺), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Near infrared dye

Antibody clone number

MAb11

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized, and then stained with TNF-α (clone MAb11) Brilliant Violet 711™ (top) or mouse IgG1, κ Brilliant Violet 711™ isotype control (bottom).

Conjugate

Red dye