502941

antibody from BioLegend

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

ELISA (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

502941

Provider

BioLegend

Proper citation

BioLegend Cat#502941, RRID:AB_2562842

Product name

Purified anti-human TNF-a (MaxPar® Ready)

Antibody type

Monoclonal

Antigen

E. coli-expressed, recombinant human TNF-α

Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4⁺), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

MAb11

Vial size

100 µg

Concentration

1 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

ELISA

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human PBMCs were incubated for 6 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 170Er-anti-CD3 (UCHT1) and 152Sm-anti-TNFα (MAb11).

Conjugate

Unconjugated