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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 502943 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#502943, RRID:AB_2562869
- Product name
- APC/Cy7 anti-human TNF-a
- Antibody type
- Monoclonal
- Antigen
- E. coli-expressed, recombinant human TNF-α
- Description
- TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- MAb11
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, fixed, permeabilized, and then stained with TNF-α (clone MAb11) APC/Cy7 (top) or mouse IgG1, κ APC/Cy7 isotype control (bottom).
