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Western blot
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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Other assay [1]
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- Product number
- MTNFAI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Monoclonal Antibody (E10043)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MTNFAI targets TNF Alpha in ELISA, and Western blot applications and shows reactivity with Human samples. The MTNFAI antibody (clone E10043) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M3TNFABI (biotinylated conjugate of clone E10041). The MTNFAI immunogen is recombinant human TNF alpha from Pichia pastoris. MTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. Endotoxin levels are less than or equal to 10 µg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- E10043
- Vial size
- 500 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Alzheimer's associated β-amyloid protein inhibits influenza A virus and modulates viral interactions with phagocytes.
Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.
Hapivirins and diprovirins: novel θ-defensin analogs with potent activity against influenza A virus.
Time-Resolved Fluorometric Method for One-Step Immunoassays Using Plasmonic Nanostructures.
White MR, Kandel R, Tripathi S, Condon D, Qi L, Taubenberger J, Hartshorn KL
PloS one 2014;9(7):e101364
PloS one 2014;9(7):e101364
Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.
Szmacinski H, Toshchakov V, Piao W, Lakowicz JR
BioNanoScience 2013 Mar 1;3(1):30-36
BioNanoScience 2013 Mar 1;3(1):30-36
Hapivirins and diprovirins: novel θ-defensin analogs with potent activity against influenza A virus.
Doss M, Ruchala P, Tecle T, Gantz D, Verma A, Hartshorn A, Crouch EC, Luong H, Micewicz ED, Lehrer RI, Hartshorn KL
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2759-68
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2759-68
Time-Resolved Fluorometric Method for One-Step Immunoassays Using Plasmonic Nanostructures.
Szmacinski H, Murtaza Z, Lakowicz JR
The journal of physical chemistry. C, Nanomaterials and interfaces 2010 Apr 29;114(16):7236-7241
The journal of physical chemistry. C, Nanomaterials and interfaces 2010 Apr 29;114(16):7236-7241
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-TNF alpha monoclonal antibody (Product # MTNFAI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 62-6520) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.
