502936

antibody from BioLegend

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

502936

Provider

BioLegend

Proper citation

BioLegend Cat#502936, RRID:AB_2563884

Product name

Brilliant Violet 605™ anti-human TNF-α

Antibody type

Monoclonal

Antigen

E. coli-expressed, recombinant human TNF-α

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

MAb11

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, fixed, permeabilized, and then stained with TNF-α (clone MAb11) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).

Conjugate

Yellow dye