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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB4101 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TNF-alpha .
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.
Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
Iron alters macrophage polarization status and leads to steatohepatitis and fibrogenesis.
Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state.
TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17.
Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice.
Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway.
Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli.
Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice.
A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection.
Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-α antibodies.
Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death.
Toll-like receptor 3 ligand dampens liver inflammation by stimulating Valpha 14 invariant natural killer T cells to negatively regulate gammadeltaT cells.
Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Shi L, Wang J, Ding N, Zhang Y, Zhu Y, Dong S, Wang X, Peng C, Zhou C, Zhou L, Li X, Shi H, Wu W, Long X, Wu C, Liao W
Nature communications 2019 Nov 28;10(1):5421
Nature communications 2019 Nov 28;10(1):5421
Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
Iron alters macrophage polarization status and leads to steatohepatitis and fibrogenesis.
Handa P, Thomas S, Morgan-Stevenson V, Maliken BD, Gochanour E, Boukhar S, Yeh MM, Kowdley KV
Journal of leukocyte biology 2019 May;105(5):1015-1026
Journal of leukocyte biology 2019 May;105(5):1015-1026
Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state.
Chhana A, Pool B, Callon KE, Tay ML, Musson D, Naot D, McCarthy G, McGlashan S, Cornish J, Dalbeth N
Arthritis research & therapy 2018 Sep 10;20(1):208
Arthritis research & therapy 2018 Sep 10;20(1):208
TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17.
Cook AD, Lee MC, Saleh R, Khiew HW, Christensen AD, Achuthan A, Fleetwood AJ, Lacey DC, Smith JE, Förster I, Hamilton JA
JCI insight 2018 Mar 22;3(6)
JCI insight 2018 Mar 22;3(6)
Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice.
Quiroga DT, Muñoz MC, Gil C, Pffeifer M, Toblli JE, Steckelings UM, Giani JF, Dominici FP
Physiological reports 2018 Aug;6(16):e13824
Physiological reports 2018 Aug;6(16):e13824
Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway.
Schock SN, Chandra NV, Sun Y, Irie T, Kitagawa Y, Gotoh B, Coscoy L, Winoto A
Cell death and differentiation 2017 Apr;24(4):615-625
Cell death and differentiation 2017 Apr;24(4):615-625
Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli.
Prasad S, Starck SR, Shastri N
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):2981-2991
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):2981-2991
Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice.
Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T
Nature medicine 2015 Sep;21(9):1085-90
Nature medicine 2015 Sep;21(9):1085-90
A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
Ruiz EJ, Oeztuerk-Winder F, Ventura JJ
Nature communications 2014;5:3175
Nature communications 2014;5:3175
MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection.
Berod L, Stüve P, Swallow M, Arnold-Schrauf C, Kruse F, Gentilini MV, Freitag J, Holzmann B, Sparwasser T
European journal of immunology 2014 May;44(5):1399-409
European journal of immunology 2014 May;44(5):1399-409
Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-α antibodies.
Bhargava R, Altmann CJ, Andres-Hernando A, Webb RG, Okamura K, Yang Y, Falk S, Schmidt EP, Faubel S
PloS one 2013;8(11):e79037
PloS one 2013;8(11):e79037
Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death.
Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, Kroemer G, Ishibashi T
The American journal of pathology 2011 Dec;179(6):2798-809
The American journal of pathology 2011 Dec;179(6):2798-809
Toll-like receptor 3 ligand dampens liver inflammation by stimulating Valpha 14 invariant natural killer T cells to negatively regulate gammadeltaT cells.
Gardner TR, Chen Q, Jin Y, Ajuebor MN
The American journal of pathology 2010 Apr;176(4):1779-89
The American journal of pathology 2010 Apr;176(4):1779-89
Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
Ismail N, Crossley EC, Stevenson HL, Walker DH
Infection and immunity 2007 Sep;75(9):4608-20
Infection and immunity 2007 Sep;75(9):4608-20
CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Cappello P, Caorsi C, Bosticardo M, De Angelis S, Novelli F, Forni G, Giovarelli M
Journal of leukocyte biology 2004 Jan;75(1):135-42
Journal of leukocyte biology 2004 Jan;75(1):135-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Recombinant Mouse TNF-alpha (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-alpha Monoclonal Antibody (Catalog # MAB4101). The ND50 is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
