AB-410-NA

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-410-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal TNF-alpha Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects mouse TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with human TNF-alpha is observed, and less than 5% cross-reactivity with recombinant bovine TNF-alpha , recombinant canine TNF-alpha , recombinant equine TNF-alpha , recombinant feline TNF-alpha , and recombinant porcine TNF-alpha is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice.

Ono Y, Maejima Y, Saito M, Sakamoto K, Horita S, Shimomura K, Inoue S, Kotani J
Scientific reports 2020 Jan 20;10(1):694

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Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing.

Mahmoudi S, Mancini E, Xu L, Moore A, Jahanbani F, Hebestreit K, Srinivasan R, Li X, Devarajan K, Prélot L, Ang CE, Shibuya Y, Benayoun BA, Chang ALS, Wernig M, Wysocka J, Longaker MT, Snyder MP, Brunet A
Nature 2019 Oct;574(7779):553-558

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Lipopolysaccharide inhibits myogenic differentiation of C2C12 myoblasts through the Toll-like receptor 4-nuclear factor-κB signaling pathway and myoblast-derived tumor necrosis factor-α.

Ono Y, Sakamoto K
PloS one 2017;12(7):e0182040

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Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.

Zhao Y, Xiao X, Frank SJ, Lin HY, Xia Y
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98

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Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha.

Hashiramoto A, Yamane T, Tsumiyama K, Yoshida K, Komai K, Yamada H, Yamazaki F, Doi M, Okamura H, Shiozawa S
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1560-5

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Long-lasting neuropathic pain induced by brachial plexus injury in mice: Role triggered by the pro-inflammatory cytokine, tumour necrosis factor alpha.

Quintão NL, Balz D, Santos AR, Campos MM, Calixto JB
Neuropharmacology 2006 Apr;50(5):614-20

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Recombinant Mouse TNF-alpha (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-alpha Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).