Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF690 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine TNF-alpha in direct ELISAs and Western blots.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
European heart journal 2008 Jun;29(12):1578-90
European heart journal 2008 Jun;29(12):1578-90
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
Atherosclerosis 2007 May;192(1):25-32
Atherosclerosis 2007 May;192(1):25-32
Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
Journal of virology 2006 Nov;80(21):10514-21
Journal of virology 2006 Nov;80(21):10514-21
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Meldrum KK, Burnett AL, Meng X, Misseri R, Shaw MB, Gearhart JP, Meldrum DR
Circulation research 2003 Feb 21;92(3):293-9
Circulation research 2003 Feb 21;92(3):293-9
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Meldrum KK, Burnett AL, Meng X, Misseri R, Shaw MB, Gearhart JP, Meldrum DR
Circulation research 2003 Feb 21;92(3):293-9
Circulation research 2003 Feb 21;92(3):293-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Porcine TNF-alpha Antibody. Recombinant Porcine TNF-alpha (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND50 is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).