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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AM32922PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Tumor necrosis factor (TNF-alpha)
- Antibody type
- Monoclonal
- Antigen
- Full-length recombinant Human TNF alpha
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- IMG6N2E12
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of TNF alpha using TNF alpha antibody at 3 µg/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human PBMC were stimulated with PMA (10 ng/ml) and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with CD3 PE antibody before testing with AF647 conjugated TNF alpha monoclonal antibody by intracellular staining. Fixed and permeabilized cells were incubated with 10 µl (0.5 µg) of TNF antibody (or mouse IgG1 antibody) for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.
