AHC3419

antibody from Invitrogen Antibodies

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

AHC3419

Provider

Invitrogen Antibodies

Product name

TNF alpha Monoclonal Antibody (68B 3C5), Biotin

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This product is recommended as a detection antibody in Sandwich ELISA applications. This clone can also be referred to as clone number 68B 3C5 4B3 A6.

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

68B 3C5

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.

Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, Le Large TYS, Driehuis E, Clevers H, Bruijns SCM, O'Toole T, van Vliet SJ, Bijlsma MF, Wuhrer M, Kazemier G, Giovannetti E, Garcia-Vallejo JJ, van Kooyk Y
Communications biology 2022 Jan 11;5(1):41

---

Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.

Inglefield JR, Larson CJ, Gibson SJ, Lebrec H, Miller RL
Journal of biomolecular screening 2006 Sep;11(6):575-85

---

Oligodeoxynucleotides differentially modulate activation of TLR7 and TLR8 by imidazoquinolines.

Gorden KK, Qiu X, Battiste JJ, Wightman PP, Vasilakos JP, Alkan SS
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):8164-70

---

Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.

Gorden KB, Gorski KS, Gibson SJ, Kedl RM, Kieper WC, Qiu X, Tomai MA, Alkan SS, Vasilakos JP
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68

---

Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.

Gorden KB, Gorski KS, Gibson SJ, Kedl RM, Kieper WC, Qiu X, Tomai MA, Alkan SS, Vasilakos JP
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68

---

Production of antibodies to canine IL-1beta and canine TNF to assess the role of proinflammatory cytokines.

Baggio V, Ott F, Fischer RW, Gram H, Peele J, Spreng D, Schmökel H, Jungi TW
Veterinary immunology and immunopathology 2005 Aug 15;107(1-2):27-39

---

The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.

Vinogradov E, Paul CJ, Li J, Zhou Y, Lyle EA, Tapping RI, Kropinski AM, Perry MB
European journal of biochemistry 2004 Dec;271(23-24):4685-95

---

Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.

Gee K, Kozlowski M, Kumar A
The Journal of biological chemistry 2003 Sep 26;278(39):37275-87

---

Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.

Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72

---

Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.

Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72

---

Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.

Braun MC, Wang JM, Lahey E, Rabin RL, Kelsall BL
Blood 2001 Jun 1;97(11):3531-6

---

Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.

Braun MC, Wang JM, Lahey E, Rabin RL, Kelsall BL
Blood 2001 Jun 1;97(11):3531-6

---

Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.

Bullens DM, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam PA, Van Gool SW, Ceuppens JL
International immunology 2001 Feb;13(2):181-91

---

Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.

Bullens DM, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam PA, Van Gool SW, Ceuppens JL
International immunology 2001 Feb;13(2):181-91

---

Selective suppression of IL-12 production by chemoattractants.

Braun MC, Lahey E, Kelsall BL
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17

---

Selective suppression of IL-12 production by chemoattractants.

Braun MC, Lahey E, Kelsall BL
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17

---

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

Braun MC, He J, Wu CY, Kelsall BL
The Journal of experimental medicine 1999 Feb 1;189(3):541-52

---

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

Braun MC, He J, Wu CY, Kelsall BL
The Journal of experimental medicine 1999 Feb 1;189(3):541-52

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 CT treatment selectively suppressed IL-12 p70 and TNF-alpha, but not IL-10, TGF-beta 1 , or IL-6. Human monocytes (2 x 10 6 cell/mL) were stimulated with SAC (0.01%) and IFN-gamma (100 ng/mL) in the presence or absence of CT (10 ng/mL). Cytokine production was measured by ELISA 24 h after stimulation. Data are mean values +- SD of four separate experiments using four different donors. ** P < 0.01.

Conjugate

Biotin