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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [24]
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- Product number
- AHC3712 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This monoclonal mixture is recommended as a capture antibody in Sandwich ELISA applications.
- Antibody clone number
- 68B2B3 and 68B6A3
- Concentration
- 1 mg/mL
Submitted references Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.
Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.
The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.
Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs.
Infliximab induces apoptosis of monocytes and T lymphocytes in a human-mouse chimeric model.
Haptoglobin dampens endotoxin-induced inflammatory effects both in vitro and in vivo.
Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.
Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.
The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.
Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
Euglycemic hyperinsulinemia augments the cytokine and endocrine responses to endotoxin in humans.
Igf-I inhibits spontaneous apoptosis in human granulocytes.
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.
Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.
Selective suppression of IL-12 production by chemoattractants.
Selective suppression of IL-12 production by chemoattractants.
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, Le Large TYS, Driehuis E, Clevers H, Bruijns SCM, O'Toole T, van Vliet SJ, Bijlsma MF, Wuhrer M, Kazemier G, Giovannetti E, Garcia-Vallejo JJ, van Kooyk Y
Communications biology 2022 Jan 11;5(1):41
Communications biology 2022 Jan 11;5(1):41
Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.
Inglefield JR, Larson CJ, Gibson SJ, Lebrec H, Miller RL
Journal of biomolecular screening 2006 Sep;11(6):575-85
Journal of biomolecular screening 2006 Sep;11(6):575-85
The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.
Njemini R, Lambert M, Demanet C, Mets T
Experimental gerontology 2006 Mar;41(3):312-9
Experimental gerontology 2006 Mar;41(3):312-9
Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs.
Kvist PH, Iburg T, Bielecki M, Gerstenberg M, Buch-Rasmussen T, Hasselager E, Jensen HE
Diabetes technology & therapeutics 2006 Aug;8(4):463-75
Diabetes technology & therapeutics 2006 Aug;8(4):463-75
Infliximab induces apoptosis of monocytes and T lymphocytes in a human-mouse chimeric model.
Shen C, Maerten P, Geboes K, Van Assche G, Rutgeerts P, Ceuppens JL
Clinical immunology (Orlando, Fla.) 2005 Jun;115(3):250-9
Clinical immunology (Orlando, Fla.) 2005 Jun;115(3):250-9
Haptoglobin dampens endotoxin-induced inflammatory effects both in vitro and in vivo.
Arredouani MS, Kasran A, Vanoirbeek JA, Berger FG, Baumann H, Ceuppens JL
Immunology 2005 Feb;114(2):263-71
Immunology 2005 Feb;114(2):263-71
Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.
Gorden KB, Gorski KS, Gibson SJ, Kedl RM, Kieper WC, Qiu X, Tomai MA, Alkan SS, Vasilakos JP
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68
Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.
Gorden KB, Gorski KS, Gibson SJ, Kedl RM, Kieper WC, Qiu X, Tomai MA, Alkan SS, Vasilakos JP
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 1;174(3):1259-68
The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.
Vinogradov E, Paul CJ, Li J, Zhou Y, Lyle EA, Tapping RI, Kropinski AM, Perry MB
European journal of biochemistry 2004 Dec;271(23-24):4685-95
European journal of biochemistry 2004 Dec;271(23-24):4685-95
Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.
Gee K, Kozlowski M, Kumar A
The Journal of biological chemistry 2003 Sep 26;278(39):37275-87
The Journal of biological chemistry 2003 Sep 26;278(39):37275-87
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
Euglycemic hyperinsulinemia augments the cytokine and endocrine responses to endotoxin in humans.
Soop M, Duxbury H, Agwunobi AO, Gibson JM, Hopkins SJ, Childs C, Cooper RG, Maycock P, Little RA, Carlson GL
American journal of physiology. Endocrinology and metabolism 2002 Jun;282(6):E1276-85
American journal of physiology. Endocrinology and metabolism 2002 Jun;282(6):E1276-85
Igf-I inhibits spontaneous apoptosis in human granulocytes.
Kooijman R, Coppens A, Hooghe-Peters E
Endocrinology 2002 Apr;143(4):1206-12
Endocrinology 2002 Apr;143(4):1206-12
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
Braun MC, Wang JM, Lahey E, Rabin RL, Kelsall BL
Blood 2001 Jun 1;97(11):3531-6
Blood 2001 Jun 1;97(11):3531-6
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
Braun MC, Wang JM, Lahey E, Rabin RL, Kelsall BL
Blood 2001 Jun 1;97(11):3531-6
Blood 2001 Jun 1;97(11):3531-6
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
Bullens DM, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam PA, Van Gool SW, Ceuppens JL
International immunology 2001 Feb;13(2):181-91
International immunology 2001 Feb;13(2):181-91
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
Bullens DM, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam PA, Van Gool SW, Ceuppens JL
International immunology 2001 Feb;13(2):181-91
International immunology 2001 Feb;13(2):181-91
Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.
Tapping RI, Akashi S, Miyake K, Godowski PJ, Tobias PS
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5780-7
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5780-7
Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.
Tapping RI, Akashi S, Miyake K, Godowski PJ, Tobias PS
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5780-7
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5780-7
Selective suppression of IL-12 production by chemoattractants.
Braun MC, Lahey E, Kelsall BL
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17
Selective suppression of IL-12 production by chemoattractants.
Braun MC, Lahey E, Kelsall BL
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3009-17
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
Braun MC, He J, Wu CY, Kelsall BL
The Journal of experimental medicine 1999 Feb 1;189(3):541-52
The Journal of experimental medicine 1999 Feb 1;189(3):541-52
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
Braun MC, He J, Wu CY, Kelsall BL
The Journal of experimental medicine 1999 Feb 1;189(3):541-52
The Journal of experimental medicine 1999 Feb 1;189(3):541-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 3 CT treatment selectively suppressed IL-12 p70 and TNF-alpha, but not IL-10, TGF-beta 1 , or IL-6. Human monocytes (2 x 10 6 cell/mL) were stimulated with SAC (0.01%) and IFN-gamma (100 ng/mL) in the presence or absence of CT (10 ng/mL). Cytokine production was measured by ELISA 24 h after stimulation. Data are mean values +- SD of four separate experiments using four different donors. ** P < 0.01.
