Explore
Validate
Learn
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6901 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects porcine TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 13% cross-reactivity with recombinant human (rh) TNF-alpha is observed and no cross-reactivity with recombinant mouse TNF-alpha , recombinant rat TNF-alpha , rhTNF-beta , rhAPRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTrance, and rhVEGF is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.
Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha.
Martin C, Waghela SD, Lokhandwala S, Ambrus A, Bray J, Vuong C, Vinodkumar V, Dominowski PJ, Rai S, Mwangi D, Foss DL, Mwangi W
PloS one 2017;12(1):e0170504
PloS one 2017;12(1):e0170504
Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha.
McNulty AL, Moutos FT, Weinberg JB, Guilak F
Arthritis and rheumatism 2007 Sep;56(9):3033-42
Arthritis and rheumatism 2007 Sep;56(9):3033-42
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Porcine TNF-alpha Antibody. Recombinant Porcine TNF-alpha (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-alpha Monoclonal Antibody (Catalog # MAB6901). The ND50 is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
