Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1011 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant cotton rat TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) EDA-A2 is observed, 5% cross-reactivity with rhTRAIL and recombinant mouse (rm) TRAIL is observed, and no cross-reactivity with rhTNF-alpha , rmTNF-alpha , rhAPRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTWEAK, rhVEGI, or rmTRANCE is observed.
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Cotton Rat TNF-alpha Antibody. Recombinant Cotton Rat TNF-alpha (Catalog # 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Cotton Rat TNF-alpha (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-alpha Monoclonal Antibody (Catalog # MAB1011). The ND50 is typically 0.05- 0.3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
