Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB410-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Hamster Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant mouse (rm)TNF-alpha in direct ELISAs and Western blots. Shows 50% cross-reactivity with rrTNF-alpha and no cross-reactivity with 17 other tested proteins.
- Reactivity
- Mouse
- Host
- Hamster
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Recombinant Mouse, and Rat TNF-alpha by Western Blot. Western blot shows 25 ng of Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT), Recombinant Human TNF-alpha (Catalog # 210-TA), Recombinant Rat TNF-alpha (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin-alpha/TNF-beta . PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-alpha Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-alpha at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3.