Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- FAB210P-050 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects cell surface expressed TNF-alpha (membrane and receptor bound forms) by flow cytometry. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TNF-alpha in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF-alpha Membrane Form PE-conjugated Monoclonal Antibody (Catalog # FAB210P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TNF- alpha in Human PBMC Monocytes by Flow Cytometry.