MAB10701

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10701

Provider

Novus Biologicals

Product name

Rat Monoclonal TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rhesus macaque TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, 10-25% cross-reactivity with recombinant human (rh) TNF-alpha and recombinant porcine TNF-alpha is observed and no cross-reactivity with rhEDA-A2, recombinant mouse (rm) EDA, rhAPRIL, rhBAFF, rhFas L, rhGITR L, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40 L, recombinant cotton rat TNF-alpha , rmTNF-alpha , recombinant rat TNF-alpha , rhTRAIL, rhTRANCE, and rhTWEAK is observed.

Host

Rat

Isotype

IgG

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Rhesus Macaque TNF-alpha Antibody. Recombinant Rhesus Macaque TNF-alpha (Catalog # 1070-RM) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rhesus Macaque TNF-alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Rhesus Macaque TNF-alpha Monoclonal Antibody (Catalog # MAB10701). The ND₅₀ is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).