Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAF1507 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine TNF-alpha Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 40% cross-reactivity with recombinant human TNF-alpha and recombinant rhesus macaque TNF-alpha is observed, less than 2% cross-reactivity with recombinant porcine (rp) TNF-alpha is observed, and less than 0.5% cross-reactivity with recombinant mouse (rm) TNF-alpha , recombinant rat (rr) TNF-alpha , recombinant cotton rat (rcr) TNF-alpha , and recombinant equine TNF-alpha is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P51742
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden.
Roatt BM, Aguiar-Soares RD, Reis LE, Cardoso JM, Mathias FA, de Brito RC, da Silva SM, Gontijo NF, Ferreira SA, Valenzuela JG, CorrĂȘa-Oliveira R, Giunchetti RC, Reis AB
Frontiers in immunology 2017;8:217
Frontiers in immunology 2017;8:217
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Canine PBMCs. TNF-alpha was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Canine TNF-alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1507) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.