BAF1507

antibody from R&D Systems

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF1507

Provider

R&D Systems

Product name

Canine TNF-alpha Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects canine TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 40% cross-reactivity with recombinant human TNF-alpha and recombinant rhesus macaque TNF-alpha is observed, less than 2% cross-reactivity with recombinant porcine (rp) TNF-alpha is observed, and less than 0.5% cross-reactivity with recombinant mouse (rm) TNF-alpha , recombinant rat (rr) TNF-alpha , recombinant cotton rat (rcr) TNF-alpha , and recombinant equine TNF-alpha is observed.

Reactivity

Canine

Host

Goat

Conjugate

Biotin

Antigen sequence

P51742


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden.

Roatt BM, Aguiar-Soares RD, Reis LE, Cardoso JM, Mathias FA, de Brito RC, da Silva SM, Gontijo NF, Ferreira SA, Valenzuela JG, Corrêa-Oliveira R, Giunchetti RC, Reis AB
Frontiers in immunology 2017;8:217

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TNF-alpha in Canine PBMCs. TNF-alpha was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Canine TNF-alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1507) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.