MAB510-100

antibody from R&D Systems

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB510-100

Provider

R&D Systems

Product name

Rat TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF-alpha and less than 0.2% cross-reactivity with rhTNF-alpha , rpTNF-alpha , and rhTNF-beta . In Western blots, this antibody shows 100% cross-reactivity with rmTNF-alpha and no cross-reactivity with rfeTNF-alpha .

Reactivity

Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P16599


Isotype

IgG

Antibody clone number

45418

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress.

Jeong JY, Chung YC, Jin BK
International journal of molecular sciences 2019 Apr 19;20(8)

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Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation.

Abd-Ellatef GF, Ahmed OM, Abdel-Reheim ES, Abdel-Hamid AZ
Breast cancer (Dove Medical Press) 2017;9:67-83

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Transport patterns of anti-TNF-α in burn wounds: Therapeutic implications of hyaluronic acid conjugation.

Friedrich EE, Washburn NR
Biomaterials 2017 Jan;114:10-22

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Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks.

Cao G, González J, Müller A, Ottaviano G, Ambrosio G, Toblli JE, Milei J
PloS one 2016;11(3):e0152461

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Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFα in vitro.

Cheng H, Davis DA, Hasheminassab S, Sioutas C, Morgan TE, Finch CE
Journal of neuroinflammation 2016 Jan 26;13:19

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Neuronal synapse formation induced by microglia and interleukin 10.

Lim SH, Park E, You B, Jung Y, Park AR, Park SG, Lee JR
PloS one 2013;8(11):e81218

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In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.

Roche M, Diamond M, Kelly JP, Finn DP
Journal of neuroimmunology 2006 Dec;181(1-2):57-67

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Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNF-alpha.

Nagakawa Y, Williams GM, Zheng Q, Tsuchida A, Aoki T, Montgomery RA, Klein AS, Sun Z
Hepatology (Baltimore, Md.) 2005 Jul;42(1):208-15

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).