Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2101-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01375
- Isotype
- IgG
- Antibody clone number
- 6401
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.
Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Cheng WC, van Asten SD, Burns LA, Evans HG, Walter GJ, Hashim A, Hughes FJ, Taams LS
European journal of immunology 2016 Sep;46(9):2211-21
European journal of immunology 2016 Sep;46(9):2211-21
Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Sommer A, Fabri M
PloS one 2015;10(6):e0130395
PloS one 2015;10(6):e0130395
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra CM, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Aróstegui JI, Juan M, Yagüe J, Merad M, Fagarasan S, Cerutti A
Nature immunology 2014 Apr;15(4):354-364
Nature immunology 2014 Apr;15(4):354-364
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
The Journal of allergy and clinical immunology 2009 Jan;123(1):239-48
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
Nature immunology 2009 Aug;10(8):857-63
Nature immunology 2009 Aug;10(8):857-63
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
Cell death and differentiation 2006 Oct;13(10):1789-801
Cell death and differentiation 2006 Oct;13(10):1789-801
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-alpha Monoclonal Antibody (Catalog # MAB2101). The ND50 is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).