Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1507 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine TNF-alpha direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant human TNF-alpha , 50% cross-reactivity with recombinant rhesus macaque TNF-alpha , 35% cross-reactivity with recombinant equine TNF-alpha , recombinant cotton rat TNF-alpha and recombinant rat TNF-alpha , 25% cross-reactivity with recombinant mouse TNF-alpha , and 10% cross-reactivity with recombinant porcine TNF-alpha is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P51742
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Canine PBMCs. TNF-alpha was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Canine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Canine TNF-alpha Antibody. Recombinant Canine TNF-alpha (Catalog # 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-alpha (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND50 is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).