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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB6903 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine TNF-alpha in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) APRIL, rhFasL, rhGITRL, rhLIGHT, recombinant mouse (rm) TRANCE, rhBAFF, rhTNF-alpha , rmTNF-alpha , recombinant rat TNF-alpha , or rhTNF-beta is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P23563- Isotype
- IgG
- Antibody clone number
- 103302
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection.
Sipos W, Duvigneau JC, Willheim M, Schilcher F, Hartl RT, Hofbauer G, Exel B, Pietschmann P, Schmoll F
Veterinary immunology and immunopathology 2004 May;99(1-2):63-71
Veterinary immunology and immunopathology 2004 May;99(1-2):63-71
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Porcine PBMCs. TNF-alpha was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Mouse Anti-Porcine TNF-alpha Monoclonal Antibody (Catalog # MAB6903) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Porcine TNF-alpha Antibody. Recombinant Porcine TNF-alpha (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Porcine TNF-alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-alpha Monoclonal Antibody (Catalog # MAB6903). The ND50 is typically 0.005-0.02 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
