Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-410-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with human TNF-alpha is observed, and less than 5% cross-reactivity with recombinant bovine TNF-alpha , recombinant canine TNF-alpha , recombinant equine TNF-alpha , recombinant feline TNF-alpha , and recombinant porcine TNF-alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P06804
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lipopolysaccharide inhibits myogenic differentiation of C2C12 myoblasts through the Toll-like receptor 4-nuclear factor-κB signaling pathway and myoblast-derived tumor necrosis factor-α.
Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.
Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha.
Long-lasting neuropathic pain induced by brachial plexus injury in mice: Role triggered by the pro-inflammatory cytokine, tumour necrosis factor alpha.
Ono Y, Sakamoto K
PloS one 2017;12(7):e0182040
PloS one 2017;12(7):e0182040
Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.
Zhao Y, Xiao X, Frank SJ, Lin HY, Xia Y
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98
Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha.
Hashiramoto A, Yamane T, Tsumiyama K, Yoshida K, Komai K, Yamada H, Yamazaki F, Doi M, Okamura H, Shiozawa S
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1560-5
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1560-5
Long-lasting neuropathic pain induced by brachial plexus injury in mice: Role triggered by the pro-inflammatory cytokine, tumour necrosis factor alpha.
Quintão NL, Balz D, Santos AR, Campos MM, Calixto JB
Neuropharmacology 2006 Apr;50(5):614-20
Neuropharmacology 2006 Apr;50(5):614-20
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Recombinant Mouse TNF-alpha (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-alpha Polyclonal Antibody (Catalog # AB-410-NA). The ND50 is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).