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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC410P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TNF-alpha PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TNF-alpha in flow cytometry.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- MP6-XT22
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line treated with anti-CD3/anti-CD28, PMA, and Calcium Ionomycin was stained with Rat Anti-Mouse TNF-alpha PE-conjugated Monoclonal Antibody (Catalog # IC410P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
