Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- NBP2-45331-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNFA/1172).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Histiocytoma stained with TNF alpha Monoclonal Antibody (TNFA/1172)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNFA/1172)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Stomach stained with TNF alpha Monoclonal Antibody (TNFA/1172)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (TNFA/1172).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using TNF-alpha Antibody (TNFA/1172).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).