Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 502931 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#502931, RRID:AB_10898321
- Product name
- Brilliant Violet 421™ anti-human TNF-รก [MAb11]
- Antibody type
- Monoclonal
- Antigen
- E. coli-expressed, recombinant human TNF-α
- Description
- TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
- Reactivity
- Human, Porcine, Simian
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses.
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, Masunaga A, Ogawa Y, Oguchi K, Miyazaki A
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1449-57
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1449-57
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in presence of monensin) were stained with CD3 FITC, fixed, permeabilized and then stained with TNF-α (clone MAb11) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
- Conjugate
- Blue dye