Antibody data
- Antibody Data
- Antigen structure
- References [113]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1079 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TNF alpha Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P, ICC/IF in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Weakening of M1 macrophage and bone resorption in periodontitis cystathionine γ-lyase-deficient mice.
Piezoelectrically-activated antibacterial catheter for prevention of urinary tract infections in an on-demand manner.
Mechanism of Salvia miltiorrhiza Bunge extract to alleviate Chronic Sleep Deprivation-Induced cognitive dysfunction in rats.
Oridonin, a small molecule inhibitor of cancer stem cell with potent cytotoxicity and differentiation potential.
NaAsO(2) regulates TLR4/MyD88/NF-κB signaling pathway through DNMT1/SOCS1 to cause apoptosis and inflammation in hepatic BRL-3A cells.
Implication of lncRNA MSTRG.81401 in Hippocampal Pyroptosis Induced by P2X7 Receptor in Type 2 Diabetic Rats with Neuropathic Pain Combined with Depression.
Robust and Multifunctional Therapeutic Nanoparticles against Peritonitis-Induced Sepsis.
Copper Ion-Inspired Dual Controllable Drug Release Hydrogels for Wound Management: Driven by Hydrogen Bonds.
Microglial SLC25A28 Deficiency Ameliorates the Brain Injury After Intracerebral Hemorrhage in Mice by Restricting Aerobic Glycolysis.
Photopolymerizable and Antibacterial Hydrogels Loaded with Metabolites from Lacticaseibacillus rhamnosus GG for Infected Wound Healing.
Uncovering the pharmacological mechanism of Shou Tai Wan on recurrent spontaneous abortion: A integrated pharmacology strategy-based research.
Dental Pulp Stem Cell-Derived Exosomes Regulate Anti-Inflammatory and Osteogenesis in Periodontal Ligament Stem Cells and Promote the Repair of Experimental Periodontitis in Rats.
Effect and mechanism of chlorogenic acid on cognitive dysfunction in mice by lipopolysaccharide-induced neuroinflammation.
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.
Intermittent fasting associated with aerobic exercise improves oxidative parameters and causes muscle damage without compromising the performance of Wistar rats.
Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway.
Hyaluronic acid-g-lipoic acid granular gel for promoting diabetic wound healing.
Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects.
Hypoxia-Induced Kidney Injury in Newborn Rats.
Suramin ameliorates osteoarthritis by acting on the Nrf2/HO-1 and NF-κB signaling pathways in chondrocytes and promoting M2 polarization in macrophages.
Regulatory Fibroblast-Like Synoviocytes Cell Membrane Coated Nanoparticles: A Novel Targeted Therapy for Rheumatoid Arthritis.
Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats.
Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway.
Identification of the BTN3A3 Gene as a Molecule Implicated in Generalized Pustular Psoriasis in a Chinese Population.
A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance.
Ameliorative effects of epigallocatechin-3-gallate nanoparticles on 2,4-dinitrochlorobenzene induced atopic dermatitis: A potential mechanism of inflammation-related necroptosis.
Activating α7nAChR ameliorates abdominal aortic aneurysm through inhibiting pyroptosis mediated by NLRP3 inflammasome.
Treatment of ulcerative colitis with Wu-Mei-Wan by inhibiting intestinal inflammatory response and repairing damaged intestinal mucosa.
Differential roles of NMDAR subunits 2A and 2B in mediating peripheral and central sensitization contributing to orofacial neuropathic pain.
Silencing P2X7R Alleviates Diabetic Neuropathic Pain Involving TRPV1 via PKCε/P38MAPK/NF-κB Signaling Pathway in Rats.
Dimethylamino group modified polydopamine nanoparticles with positive charges to scavenge cell-free DNA for rheumatoid arthritis therapy.
Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration.
Investigation of Lonicera japonica Flos against Nonalcoholic Fatty Liver Disease Using Network Integration and Experimental Validation.
Hyperbaric oxygen therapy mitigates left ventricular remodeling, upregulates MMP-2 and VEGF, and inhibits the induction of MMP-9, TGF-β1, and TNF-α in streptozotocin-induced diabetic rat heart.
Shikonin protects against lipopolysaccharide-induced inflammation and apoptosis in human nucleus pulposus cells through the nuclear factor-kappa B pathway.
Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.
Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.
Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora.
SHP-1 ameliorates nonalcoholic steatohepatitis by inhibiting proinflammatory cytokine production.
Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer's disease.
Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.
Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy.
The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice.
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis.
Bioconjugated Carbon Dots for Delivery of siTnfα to Enhance Chondrogenesis of Mesenchymal Stem Cells by Suppression of Inflammation.
Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress.
Tetramethylpyrazine (TMP) ameliorates corneal neovascularization via regulating cell infiltration into cornea after alkali burn.
Total flavonoids, extracted from Polygonum knotweed L, exert beneficial hepatoprotection against liver injury.
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Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways.
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Lactoferrin deficiency promotes colitis-associated colorectal dysplasia in mice.
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Ulinastatin attenuates renal interstitial inflammation and inhibits fibrosis progression in rats under unilateral ureteral obstruction.
Montelukast reduces sepsis-induced lung and renal injury in rats.
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Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury.
The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation.
T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease.
Quantitative evaluation of biological reaction kinetics in confined nanospaces.
Sensitive immunosensor for tumor necrosis factor α based on dual signal amplification of ferrocene modified self-assembled peptide nanowire and glucose oxidase functionalized gold nanorod.
Expression change of TNF-α in myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopolysaccharide.
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Label-free electrochemical immunosensors based on surface-initiated atom radical polymerization.
Effect of renal denervation procedure on left ventricular hypertrophy of hypertensive rats and its mechanisms.
Melanocortin 4 receptor antagonists attenuates morphine antinociceptive tolerance, astroglial activation and cytokines expression in the spinal cord of rat.
Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats.
Prostate extract with aluminum hydroxide injection as a novel animal model for chronic prostatitis/chronic pelvic pain syndrome.
Bone marrow mesenchymal stem cells in a three-dimensional gelatin sponge scaffold attenuate inflammation, promote angiogenesis, and reduce cavity formation in experimental spinal cord injury.
Polymer-functionalized silica nanosphere labels for ultrasensitive detection of tumor necrosis factor-alpha.
Sophocarpine alleviates non-alcoholic steatohepatitis in rats.
Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye.
Protective effects of icariin on brain dysfunction induced by lipopolysaccharide in rats.
Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma.
Interleukin-15 receptor-directed immunotoxins atteunuate disease severity in rat adjuvant arthritis.
Hypoxia influences linearly patterned programmed cell necrosis and tumor blood supply patterns formation in melanoma.
Protective effect of JBP485 on concanavalin A-induced liver injury in mice.
Tumor necrosis factor-α of Red nucleus involved in the development of neuropathic allodynia.
Protective effect of bicyclol on acute alcohol-induced liver injury in mice.
Lycopene from tomatoes partially alleviates the bleomycin-induced experimental pulmonary fibrosis in rats.
Correlation between omental TNF-alpha protein and plasma PAI-1 in obesity subjects.
Radiation-induced apoptosis along with local and systemic cytokine elaboration is associated with DC plus radiotherapy-mediated renal cell tumor regression.
Infrequent COX-2 expression due to promoter hypermethylation in gastric cancers in Dalian, China.
Curcumin ameliorates left ventricular function in rabbits with pressure overload: inhibition of the remodeling of the left ventricular collagen network associated with suppression of myocardial tumor necrosis factor-alpha and matrix metalloproteinase-2 expression.
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Effect and mechanism of chlorogenic acid on cognitive dysfunction in mice by lipopolysaccharide-induced neuroinflammation.
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Activating α7nAChR ameliorates abdominal aortic aneurysm through inhibiting pyroptosis mediated by NLRP3 inflammasome.
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Silencing P2X7R Alleviates Diabetic Neuropathic Pain Involving TRPV1 via PKCε/P38MAPK/NF-κB Signaling Pathway in Rats.
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Dimethylamino group modified polydopamine nanoparticles with positive charges to scavenge cell-free DNA for rheumatoid arthritis therapy.
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SHP-1 ameliorates nonalcoholic steatohepatitis by inhibiting proinflammatory cytokine production.
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Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer's disease.
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Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.
Sun S, Wang Y, Du Y, Sun Q, He L, Zhu E, Li J
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Pharmaceutical biology 2020 Dec;58(1):1277-1289
Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy.
Zhong G, Yang X, Jiang X, Kumar A, Long H, Xie J, Zheng L, Zhao J
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Nanoscale 2019 Jun 20;11(24):11605-11616
The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice.
Ding L, Cheng P, Wang L, Hu J, Zhang YX, Cai GW, Huang GY, Gao S
BMC complementary and alternative medicine 2019 Jun 13;19(1):127
BMC complementary and alternative medicine 2019 Jun 13;19(1):127
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis.
Zhong G, Long H, Ma S, Shunhan Y, Li J, Yao J
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Life sciences 2019 Jun 1;226:164-172
Bioconjugated Carbon Dots for Delivery of siTnfα to Enhance Chondrogenesis of Mesenchymal Stem Cells by Suppression of Inflammation.
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Stem cells translational medicine 2019 Jul;8(7):724-736
Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress.
Su M, Liang X, Xu X, Wu X, Yang B
Environmental toxicology and pharmacology 2019 Jan;65:60-65
Environmental toxicology and pharmacology 2019 Jan;65:60-65
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- Main image
- Experimental details
- Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HL-60 whole cell lysates. Lane 5: human A431 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human K562 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 28KD. The expected band size for TNF alpha is at 25KD.
- Additional image