PP1071P1

antibody from Acris Antibodies GmbH

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Functional assay (Recommended by provider)

Antibody Data

Product number

PP1071P1

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#PP1071P1, RRID:AB_1008136

Product name

anti Tumor necrosis factor (TNF-alpha)

Antibody type

Polyclonal

Antigen

Highly pure (>98%) E.coli derived recombinant Human TNF-alpha

Reactivity

Human

Host

Rabbit

Vial size

50 µg

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: This TNF-alpha antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 µg/ml-1.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.