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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-210-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant canine TNF-alpha , recombinant bovine TNF-alpha , recombinant rat TNF-alpha , recombinant equine TNF-alpha , and recombinant cotton rat TNF-alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01375- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
Antigenic stimulation in the simian model of HIV infection yields dilated cardiomyopathy through effects of TNFalpha.
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Liang X, Moseman EA, Farrar MA, Bachanova V, Weisdorf DJ, Blazar BR, Chen W
Blood 2010 Jun 17;115(24):5041-52
Blood 2010 Jun 17;115(24):5041-52
Antigenic stimulation in the simian model of HIV infection yields dilated cardiomyopathy through effects of TNFalpha.
Yearley JH, Mansfield KG, Carville AA, Sokos GG, Xia D, Pearson CB, Shannon RP
AIDS (London, England) 2008 Mar 12;22(5):585-94
AIDS (London, England) 2008 Mar 12;22(5):585-94
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Kinasewitz GT, Chang AC, Peer GT, Hinshaw LB, Taylor FB Jr
Shock (Augusta, Ga.) 2000 Feb;13(2):100-9
Shock (Augusta, Ga.) 2000 Feb;13(2):100-9
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Kinasewitz GT, Chang AC, Peer GT, Hinshaw LB, Taylor FB Jr
Shock (Augusta, Ga.) 2000 Feb;13(2):100-9
Shock (Augusta, Ga.) 2000 Feb;13(2):100-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-alpha Polyclonal Antibody (Catalog # AB-210-NA). The ND50 is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
