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- Antigen structure
- References [30]
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- Product number
- BAF210 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) TNF-beta , recombinant mouse (rm) TNF-alpha , recombinant rat TNF-alpha , recombinant porcine TNF-alpha , rhsTNF RII, rmsTNF RI, and rmsTNF RII is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P01375- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma.
Transmembrane TNF-dependent uptake of anti-TNF antibodies.
Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils.
Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Protection from RNA and DNA viruses by IL-32.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Sepsis and pathophysiology of anthrax in a nonhuman primate model.
Selective regulation of IL-10 signaling and function by zymosan.
Selective regulation of IL-10 signaling and function by zymosan.
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
trans fatty acids and systemic inflammation in heart failure.
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Brencicova E, Jagger AL, Evans HG, Georgouli M, Laios A, Attard Montalto S, Mehra G, Spencer J, Ahmed AA, Raju-Kankipati S, Taams LS, Diebold SS
PloS one 2017;12(4):e0175712
PloS one 2017;12(4):e0175712
Transmembrane TNF-dependent uptake of anti-TNF antibodies.
Deora A, Hegde S, Lee J, Choi CH, Chang Q, Lee C, Eaton L, Tang H, Wang D, Lee D, Michalak M, Tomlinson M, Tao Q, Gaur N, Harvey B, McLoughlin S, Labkovsky B, Ghayur T
mAbs 2017 May Jun;9(4):680-695
mAbs 2017 May Jun;9(4):680-695
Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils.
Castro SA, Collighan R, Lambert PA, Dias IH, Chauhan P, Bland CE, Milic I, Milward MR, Cooper PR, Devitt A
Cell death & disease 2017 Mar 2;8(3):e2644
Cell death & disease 2017 Mar 2;8(3):e2644
Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
Matt P, Lindqvist U, Kleinau S
PloS one 2015;10(9):e0137474
PloS one 2015;10(9):e0137474
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia 2011 Oct;54(10):2713-23
Diabetologia 2011 Oct;54(10):2713-23
Protection from RNA and DNA viruses by IL-32.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2008 Apr;38(4):594-601
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2008 Apr;38(4):594-601
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clinical chemistry 2007 Nov;53(11):2010-2
Clinical chemistry 2007 Nov;53(11):2010-2
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clinical chemistry 2007 Nov;53(11):2010-2
Clinical chemistry 2007 Nov;53(11):2010-2
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Wunder DM, Mueller MD, Birkhäuser MH, Bersinger NA
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Acta obstetricia et gynecologica Scandinavica 2006;85(3):336-42
Sepsis and pathophysiology of anthrax in a nonhuman primate model.
Stearns-Kurosawa DJ, Lupu F, Taylor FB Jr, Kinasewitz G, Kurosawa S
The American journal of pathology 2006 Aug;169(2):433-44
The American journal of pathology 2006 Aug;169(2):433-44
Selective regulation of IL-10 signaling and function by zymosan.
Du Z, Kelly E, Mecklenbräuker I, Agle L, Herrero C, Paik P, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
Selective regulation of IL-10 signaling and function by zymosan.
Du Z, Kelly E, Mecklenbräuker I, Agle L, Herrero C, Paik P, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4785-92
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
Sharif MN, Tassiulas I, Hu Y, Mecklenbräuker I, Tarakhovsky A, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
Sharif MN, Tassiulas I, Hu Y, Mecklenbräuker I, Tarakhovsky A, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6476-81
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Human PBMCs. TNF-alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF-alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
