MA5-18728

antibody from Invitrogen Antibodies

Targeting: SEPTIN1 DIFF6, PNUTL3, SEPT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18728

Provider

Invitrogen Antibodies

Product name

Septin 1 Monoclonal Antibody (1F12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: MDKEYVGFAA LPNQLHRKSV KKGFDFTLMV AGESGLGKST LINSLFLTNL YEDRQVPEAS ARLTQTLAIE RRGVEIEEGG VKVKLTLVDT PGFGDSVDCS DCWLPVVKFI EEQFEQYLRD ESGLNRKNIQ DSRVHCCLYF ISPFGRGLRP LDVAFLRAVH EKVNIIPVIG KADALMPQET QALKQKIRDQ LKEEEIHIYQ FPECDSDEDE DFKRQDAEMK ESIPFAVVGS CEVVRDGGNR PVRGRRYSWG TVEVENPHHC DFLNLRRMLV QTHLQDLKEV THDLLYEGYR ARRLQSLARP GARDRASRSK LSRQSATEIP LPMLPLADTE KLIREKDEEL RRMQEMLEKM QAQMQRSQAQ GEQSDAL

Antibody clone number

1F12

Concentration

0.2-1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEPT1 expressed in transfected 293T cell line by anti-SEPT1 monoclonal antibody (Product # MA5-18728), clone 1F12. Lane 1: SEPT1 transfected lysate (42 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using an anti-SEPT1 monoclonal antibody (Product # MA5-18728). (antibody concentration 10 µg/mL).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody (Product # MA5-18728) and Protein A Magnetic Beads, and immunoblotted with SEPT1 rabbit polyclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SEPT1 using anti-SEPT1 monoclonal antibody (Product # MA5-18728) is 3 ng/mL as a capture antibody.