Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00001731-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001731-M03, RRID:AB_1146963
- Product name
- Mouse Monoclonal Septin-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SEPT1 - septin 1 (1F12)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12. Lane 1: SEPT1 transfected lysatE (42 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Septin-1 Antibody (1F12) [H00001731-M03] - Detection limit for recombinant GST tagged SEPT1 is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 rabbit polyclonal antibody.