H00001731-M03

antibody from Invitrogen Antibodies

Targeting: SEPTIN1 DIFF6, PNUTL3, SEPT1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001731-M03

Provider

Invitrogen Antibodies

Product name

SEPT1 Monoclonal Antibody (1F12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MDKEYVGFAA LPNQLHRKSV KKGFDFTLMV AGESGLGKST LINSLFLTNL YEDRQVPEAS ARLTQTLAIE RRGVEIEEGG VKVKLTLVDT PGFGDSVDCS DCWLPVVKFI EEQFEQYLRD ESGLNRKNIQ DSRVHCCLYF ISPFGRGLRP LDVAFLRAVH EKVNIIPVIG KADALMPQET QALKQKIRDQ LKEEEIHIYQ FPECDSDEDE DFKRQDAEMK ESIPFAVVGS CEVVRDGGNR PVRGRRYSWG TVEVENPHHC DFLNLRRMLV QTHLQDLKEV THDLLYEGYR ARRLQSLARP GARDRASRSK LSRQSATEIP LPMLPLADTE KLIREKDEEL RRMQEMLEKM QAQMQRSQAQ GEQSDAL

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F12

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12.Lane 1: SEPT1 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12.Lane 1: SEPT1 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SEPT1 is 3 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration is 10 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 MaxPab rabbit polyclonal antibody.