PAB2744

antibody from Abnova Corporation

Targeting: RFK FLJ11149, RIFK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2744

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2744, RRID:AB_1579715

Product name

RFK polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RFK.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism.

Karthikeyan S, Zhou Q, Osterman AL, Zhang H
Biochemistry 2003 Nov 4;42(43):12532-8

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Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch.

Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H
Structure (London, England : 1993) 2003 Mar;11(3):265-73

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of RFK (arrow) using RFK polyclonal antibody (Cat # PAB2744).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RFK gene (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with RFK polyclonal antibody (Cat # PAB2744) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)