NBP2-16908

antibody from Novus Biologicals

Targeting: AIF1 AIF-1, Em:AF129756.17, IBA1, IRT-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16908

Provider

Novus Biologicals

Product name

Rabbit Polyclonal AIF-1/Iba1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Devising micro/nano-architectures in multi-channel nerve conduits towards a pro-regenerative matrix for the repair of spinal cord injury.

Sun X, Bai Y, Zhai H, Liu S, Zhang C, Xu Y, Zou J, Wang T, Chen S, Zhu Q, Liu X, Mao H, Quan D
Acta biomaterialia 2019 Mar 1;86:194-206

---

Annexin A1-derived peptide Ac(2-26) in a pilocarpine-induced status epilepticus model: anti-inflammatory and neuroprotective effects.

Gimenes AD, Andrade BFD, Pinotti JVP, Oliani SM, Galvis-Alonso OY, Gil CD
Journal of neuroinflammation 2019 Feb 12;16(1):32

---

Pharmacogenetic neuronal stimulation increases human tau pathology and trans-synaptic spread of tau to distal brain regions in mice.

Schultz MK Jr, Gentzel R, Usenovic M, Gretzula C, Ware C, Parmentier-Batteur S, Schachter JB, Zariwala HA
Neurobiology of disease 2018 Oct;118:161-176

---

Purinergic P2Y1 Receptors Control Rapid Expression of Plasma Membrane Processes in Hippocampal Astrocytes.

Chisari M, Scuderi A, Ciranna L, Volsi GL, Licata F, Sortino MA
Molecular neurobiology 2017 Aug;54(6):4081-4093

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various whole 30 ug cell extracts (Molecular weight: 16.7 KDa) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at a dilution of 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted by 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5ug of Iba1 antibody Western blot analysis was performed using Iba1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded breast cancer stroma. Iba1 antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-16908] - Frozen sectioned E13.5 Rat brain. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded mouse brain.Iba1 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded rat brain stained using AIF-1/Iba1 Antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse thymus gland stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.