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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [9]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- GTX100042 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100042, RRID:AB_1240434
- Product name
- Iba1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Increased expression of endoplasmic reticulum stress-related caspase-12 and CHOP in the hippocampus of EAE mice.
Naturally occurring autoantibodies against α-synuclein rescues memory and motor deficits and attenuates α-synuclein pathology in mouse model of Parkinson's disease.
Intravenous immunoglobulin ameliorates motor and cognitive deficits and neuropathology in R6/2 mouse model of Huntington's disease by decreasing mutant huntingtin protein level and normalizing NF-κB signaling pathway.
Anti-TNF-α restricts dengue virus-induced neuropathy.
Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice.
NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation.
Sulfotransferase 4A1 Increases Its Expression in Mouse Neurons as They Mature.
Hepatitis B core VLP-based mis-disordered tau vaccine elicits strong immune response and alleviates cognitive deficits and neuropathology progression in Tau.P301S mouse model of Alzheimer's disease and frontotemporal dementia.
Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.
Neuroprotective effects of pifithrin-α against traumatic brain injury in the striatum through suppression of neuroinflammation, oxidative stress, autophagy, and apoptosis.
Aging exacerbates cognitive and anxiety alterations induced by an intracerebroventricular injection of amyloid-β1-42 peptide in mice.
Gamma-Secretase Inhibitors Attenuate Neurotrauma and Neurogenic Acute Lung Injury in Rats by Rescuing the Accumulation of Hypertrophic Microglia.
Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Long-term enzyme replacement therapy improves neurocognitive functioning and hippocampal synaptic plasticity in immune-tolerant alpha-mannosidosis mice.
PLGA nanoparticles modified with a BBB-penetrating peptide co-delivering Aβ generation inhibitor and curcumin attenuate memory deficits and neuropathology in Alzheimer's disease mice.
Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages.
A vaccine with Aβ oligomer-specific mimotope attenuates cognitive deficits and brain pathologies in transgenic mice with Alzheimer's disease.
A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease.
A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses.
Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway.
Ataxia is the major neuropathological finding in arylsulfatase G-deficient mice: similarities and dissimilarities to Sanfilippo disease (mucopolysaccharidosis type III).
Kamarehei M, Kabudanian Ardestani S, Firouzi M, Zahednasab H, Keyvani H, Harirchian MH
Brain research bulletin 2019 Apr;147:174-182
Brain research bulletin 2019 Apr;147:174-182
Naturally occurring autoantibodies against α-synuclein rescues memory and motor deficits and attenuates α-synuclein pathology in mouse model of Parkinson's disease.
Huang YR, Xie XX, Ji M, Yu XL, Zhu J, Zhang LX, Liu XG, Wei C, Li G, Liu RT
Neurobiology of disease 2019 Apr;124:202-217
Neurobiology of disease 2019 Apr;124:202-217
Intravenous immunoglobulin ameliorates motor and cognitive deficits and neuropathology in R6/2 mouse model of Huntington's disease by decreasing mutant huntingtin protein level and normalizing NF-κB signaling pathway.
Liu SY, Yu XL, Zhu J, Liu XM, Zhang Y, Dong QX, Ma S, Liu RT
Brain research 2018 Oct 15;1697:21-33
Brain research 2018 Oct 15;1697:21-33
Anti-TNF-α restricts dengue virus-induced neuropathy.
Jhan MK, HuangFu WC, Chen YF, Kao JC, Tsai TT, Ho MR, Shen TJ, Tseng PC, Wang YT, Lin CF
Journal of leukocyte biology 2018 Nov;104(5):961-968
Journal of leukocyte biology 2018 Nov;104(5):961-968
Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice.
Wahl D, Solon-Biet SM, Wang QP, Wali JA, Pulpitel T, Clark X, Raubenheimer D, Senior AM, Sinclair DA, Cooney GJ, de Cabo R, Cogger VC, Simpson SJ, Le Couteur DG
Cell reports 2018 Nov 20;25(8):2234-2243.e6
Cell reports 2018 Nov 20;25(8):2234-2243.e6
NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation.
Huang WY, Lin S, Chen HY, Chen YP, Chen TY, Hsu KS, Wu HM
Journal of neuroinflammation 2018 May 12;15(1):140
Journal of neuroinflammation 2018 May 12;15(1):140
Sulfotransferase 4A1 Increases Its Expression in Mouse Neurons as They Mature.
Hashiguchi T, Shindo S, Chen SH, Hong JS, Negishi M
Drug metabolism and disposition: the biological fate of chemicals 2018 Jun;46(6):860-864
Drug metabolism and disposition: the biological fate of chemicals 2018 Jun;46(6):860-864
Hepatitis B core VLP-based mis-disordered tau vaccine elicits strong immune response and alleviates cognitive deficits and neuropathology progression in Tau.P301S mouse model of Alzheimer's disease and frontotemporal dementia.
Ji M, Xie XX, Liu DQ, Yu XL, Zhang Y, Zhang LX, Wang SW, Huang YR, Liu RT
Alzheimer's research & therapy 2018 Jun 19;10(1):55
Alzheimer's research & therapy 2018 Jun 19;10(1):55
Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.
Ingold I, Berndt C, Schmitt S, Doll S, Poschmann G, Buday K, Roveri A, Peng X, Porto Freitas F, Seibt T, Mehr L, Aichler M, Walch A, Lamp D, Jastroch M, Miyamoto S, Wurst W, Ursini F, Arnér ESJ, Fradejas-Villar N, Schweizer U, Zischka H, Friedmann Angeli JP, Conrad M
Cell 2018 Jan 25;172(3):409-422.e21
Cell 2018 Jan 25;172(3):409-422.e21
Neuroprotective effects of pifithrin-α against traumatic brain injury in the striatum through suppression of neuroinflammation, oxidative stress, autophagy, and apoptosis.
Huang YN, Yang LY, Greig NH, Wang YC, Lai CC, Wang JY
Scientific reports 2018 Feb 5;8(1):2368
Scientific reports 2018 Feb 5;8(1):2368
Aging exacerbates cognitive and anxiety alterations induced by an intracerebroventricular injection of amyloid-β1-42 peptide in mice.
Souza LC, Jesse CR, Del Fabbro L, de Gomes MG, Gomes NS, Filho CB, Goes ATR, Wilhelm EA, Luchese C, Roman SS, Boeira SP
Molecular and cellular neurosciences 2018 Apr;88:93-106
Molecular and cellular neurosciences 2018 Apr;88:93-106
Gamma-Secretase Inhibitors Attenuate Neurotrauma and Neurogenic Acute Lung Injury in Rats by Rescuing the Accumulation of Hypertrophic Microglia.
Lin HJ, Hsu CC, Chio CC, Tian YF, Lin MT, Lin TW, Chang CH, Chang CP
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):1726-1740
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):1726-1740
Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Zera K, Zastre J
PloS one 2017;12(10):e0186707
PloS one 2017;12(10):e0186707
Long-term enzyme replacement therapy improves neurocognitive functioning and hippocampal synaptic plasticity in immune-tolerant alpha-mannosidosis mice.
Stroobants S, Damme M, Van der Jeugd A, Vermaercke B, Andersson C, Fogh J, Saftig P, Blanz J, D'Hooge R
Neurobiology of disease 2017 Oct;106:255-268
Neurobiology of disease 2017 Oct;106:255-268
PLGA nanoparticles modified with a BBB-penetrating peptide co-delivering Aβ generation inhibitor and curcumin attenuate memory deficits and neuropathology in Alzheimer's disease mice.
Huang N, Lu S, Liu XG, Zhu J, Wang YJ, Liu RT
Oncotarget 2017 Oct 6;8(46):81001-81013
Oncotarget 2017 Oct 6;8(46):81001-81013
Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages.
Lin W, Hsuan YC, Lin MT, Kuo TW, Lin CH, Su YC, Niu KC, Chang CP, Lin HJ
Cell transplantation 2017 Nov;26(11):1798-1810
Cell transplantation 2017 Nov;26(11):1798-1810
A vaccine with Aβ oligomer-specific mimotope attenuates cognitive deficits and brain pathologies in transgenic mice with Alzheimer's disease.
Wang SW, Liu DQ, Zhang LX, Ji M, Zhang YX, Dong QX, Liu SY, Xie XX, Liu RT
Alzheimer's research & therapy 2017 Jun 7;9(1):41
Alzheimer's research & therapy 2017 Jun 7;9(1):41
A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease.
Wolf H, Damme M, Stroobants S, D'Hooge R, Beck HC, Hermans-Borgmeyer I, Lüllmann-Rauch R, Dierks T, Lübke T
Disease models & mechanisms 2016 Sep 1;9(9):1015-28
Disease models & mechanisms 2016 Sep 1;9(9):1015-28
A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses.
Zha J, Liu XM, Zhu J, Liu SY, Lu S, Xu PX, Yu XL, Liu RT
Scientific reports 2016 Nov 8;6:36631
Scientific reports 2016 Nov 8;6:36631
Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway.
Liu HS, Shi HL, Huang F, Peterson KE, Wu H, Lan YY, Zhang BB, He YX, Woods T, Du M, Wu XJ, Wang ZT
Scientific reports 2016 Jan 11;6:19137
Scientific reports 2016 Jan 11;6:19137
Ataxia is the major neuropathological finding in arylsulfatase G-deficient mice: similarities and dissimilarities to Sanfilippo disease (mucopolysaccharidosis type III).
Kowalewski B, Heimann P, Ortkras T, Lüllmann-Rauch R, Sawada T, Walkley SU, Dierks T, Damme M
Human molecular genetics 2015 Apr 1;24(7):1856-68
Human molecular genetics 2015 Apr 1;24(7):1856-68
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) 15% SDS PAGE AIF1 antibody GTX100042 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein by Western blot analysis.A. 50 µg mouse brain lysate/extract15 % SDS-PAGEIba1 antibody (GTX100042) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein by western blot analysis.A. 30 ?g THP-1 whole cell lysate/extract B. 30 ?g HL-60 whole cell lysate/extract15% SDS-PAGEIba1 antibody (GTX100042) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein by Western blot analysis.A. 30 £gg THP-1 whole cell lysate/extractB. 30 £gg HL-60 whole cell lysate/extract15 % SDS-PAGEIba1 antibody (GTX100042) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein by Western blot analysis.A. 50 £gg mouse brain lysate/extract15 % SDS-PAGEIba1 antibody (GTX100042) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:400.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain tissue. Iba1 (GTX100042) dilution: 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma stroma. Iba1 antibody (GTX100042) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Iba1 antibody (GTX100042) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Iba1 antibody (GTX100042) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Iba1 antibody (GTX100042) detects Iba1 by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody (GTX100042) dilution: 1:50.
