Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN950443 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Adaptor-Related Protein Complex 1, mu 1 Subunit (AP1M1) (AA 206-235), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 206-235 amino acids from the Central region of human AP1M1
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 206-235,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Human kidney anion exchanger 1 interacts with adaptor-related protein complex 1 μ1A (AP-1 mu1A).
An empirical framework for binary interactome mapping.
AP-1 membrane-cytoplasm recycling regulated by mu1A-adaptin.
Cooperative binding of the class I major histocompatibility complex cytoplasmic domain and human immunodeficiency virus type 1 Nef to the endosomal AP-1 complex via its mu subunit.
HIV-1 Nef disrupts MHC-I trafficking by recruiting AP-1 to the MHC-I cytoplasmic tail.
Sawasdee N, Junking M, Ngaojanlar P, Sukomon N, Ungsupravate D, Limjindaporn T, Akkarapatumwong V, Noisakran S, Yenchitsomanus PT
Biochemical and biophysical research communications 2010 Oct 8;401(1):85-91
Biochemical and biophysical research communications 2010 Oct 8;401(1):85-91
An empirical framework for binary interactome mapping.
Venkatesan K, Rual JF, Vazquez A, Stelzl U, Lemmens I, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barabási AL, Vidal M
Nature methods 2009 Jan;6(1):83-90
Nature methods 2009 Jan;6(1):83-90
AP-1 membrane-cytoplasm recycling regulated by mu1A-adaptin.
Medigeshi GR, Krikunova M, Radhakrishnan K, Wenzel D, Klingauf J, Schu P
Traffic (Copenhagen, Denmark) 2008 Jan;9(1):121-32
Traffic (Copenhagen, Denmark) 2008 Jan;9(1):121-32
Cooperative binding of the class I major histocompatibility complex cytoplasmic domain and human immunodeficiency virus type 1 Nef to the endosomal AP-1 complex via its mu subunit.
Noviello CM, Benichou S, Guatelli JC
Journal of virology 2008 Feb;82(3):1249-58
Journal of virology 2008 Feb;82(3):1249-58
HIV-1 Nef disrupts MHC-I trafficking by recruiting AP-1 to the MHC-I cytoplasmic tail.
Roeth JF, Williams M, Kasper MR, Filzen TM, Collins KL
The Journal of cell biology 2004 Dec 6;167(5):903-13
The Journal of cell biology 2004 Dec 6;167(5):903-13
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP1M1 Antibody (Center) (Cat. #AP50199PU-N) western blot analysis in A375 cell line lysates (35μg/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP1M1 Antibody (Center) (Cat. #AP50199PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.