OAAB03423

antibody from Aviva Systems Biology

Targeting: EDA ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, ODT1, XHED, XLHED

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB03423

Provider

Aviva Systems Biology

Product name

EDA antibody - N-terminal region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of anti-EDA Antibody (N-term) (RB13883) in Hela cell lysates (35ug/lane). EDA(arrow) was detected using the purified Pab (1:60 dilution).

Sample type

Hela cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Sample type

human hepatocarcinoma tissue

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

hela cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol