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LearnPAB4866
antibody from Abnova Corporation
Targeting: EDA
ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, ODT1, XHED, XLHED
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4866 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4866, RRID:AB_1574544
- Product name
- EDA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EDA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A novel 4-bp insertion mutation in EDA1 gene in a Pakistani family with X-linked hypohidrotic ectodermal dysplasia.
A novel Gln358Glu mutation in ectodysplasin A associated with X-linked dominant incisor hypodontia.
Tariq M, Wasif N, Ayub M, Ahmad W
European journal of dermatology : EJD 2007 May-Jun;17(3):209-12
European journal of dermatology : EJD 2007 May-Jun;17(3):209-12
A novel Gln358Glu mutation in ectodysplasin A associated with X-linked dominant incisor hypodontia.
Tarpey P, Pemberton TJ, Stockton DW, Das P, Ninis V, Edkins S, Andrew Futreal P, Wooster R, Kamath S, Nayak R, Stratton MR, Patel PI
American journal of medical genetics. Part A 2007 Feb 15;143(4):390-4
American journal of medical genetics. Part A 2007 Feb 15;143(4):390-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EDA polyclonal antibody (Cat # PAB4866) in HeLa cell lysate (35 ug/lane). EDA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with EDA polyclonal antibody (Cat # PAB4866) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EDA polyclonal antibody (Cat # PAB4866)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
