PAB29451

antibody from Abnova Corporation

Targeting: BAG6 BAT3, D6S52E, G3, Scythe

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29451

Provider

Abnova Corporation

Product name

BAT3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human BAT3.

Antigen sequence

SDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKA
AGARPLTSPESLSRDLEAPEVQESYRQQLRSDIQK
RLQEDPNYS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with BAT3 polyclonal antibody (Cat # PAB29451) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with BAT3 polyclonal antibody (Cat # PAB29451) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with BAT3 polyclonal antibody (Cat # PAB29451) shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)