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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-53585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLSTD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YYEGKNVLLT GATGFLGKVL LEKLLRSCPK VNSVYVLVRQ KAGQTPQERV EEVLSGKLFD RLRDENPDFR EKIIAINSEL TQPKLALSEE DKEVIIDSTN IIFHCAATVR FNENLRDAVQ LNVIATRQLI LLAQQMKNLE VFM
- Concentration
- 0.10 mg/mL
Submitted references Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.
Bennett CF, O'Malley KE, Perry EA, Balsa E, Latorre-Muro P, Riley CL, Luo C, Jedrychowski M, Gygi SP, Puigserver P
Nature chemical biology 2021 Jun;17(6):703-710
Nature chemical biology 2021 Jun;17(6):703-710
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MLSTD2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MLSTD2 Polyclonal Antibody (Product # PA5-53585).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MLSTD2 in human cell line RT4 using a MLSTD2 Polyclonal Antibody (Product # PA5-53585) shows localization to peroxisomes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLSTD2 in human duodenum tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a MLSTD2 Polyclonal Antibody (Product # PA5-53585).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Fig. 6 Ether lipid synthesis is required for respirasome assembly from DHODH inhibition a , Western blot of FAR1 from cells treated with Brequinar in the presence or absence of uridine (n=2 experiments). b , Western blot of FAR1 from sgFAR1 cells (n=2 experiments). c , Lipidomic analysis of PC and PE subclass abundances in mitochondrial fractions isolated from sgFAR1 cells treated with Brequinar (min-max box plot, line at mean, n=3 biologically independent samples, Student's t-test with a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 5%, * left to right sgControl DMSO [compared to sgControl Breq] q= 0.0003, 0.0034, 0.0007, sgControl DMSO [compared to sgFAR1 DMSO] q =0.0208,
