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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29077 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDKAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Juarkat, Raji, K562, NIH-3T3.
- Concentration
- 1 mg/mL
Submitted references Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification.
Santos MCFD, Anderson CP, Neschen S, Zumbrennen-Bullough KB, Romney SJ, Kahle-Stephan M, Rathkolb B, Gailus-Durner V, Fuchs H, Wolf E, Rozman J, de Angelis MH, Cai WM, Rajan M, Hu J, Dedon PC, Leibold EA
Nature communications 2020 Jan 15;11(1):296
Nature communications 2020 Jan 15;11(1):296
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDKAL1 Polyclonal Antibody detects CDKAL1 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. CDKAL1 Polyclonal Antibody (Product # PA5-29077) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CDKAL1 Polyclonal Antibody (Product # PA5-29077). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. CDKAL1 Polyclonal Antibody (Product # PA5-29077) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDKAL1 Polyclonal Antibody detects CDKAL1 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. CDKAL1 Polyclonal Antibody (Product # PA5-29077) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using CDKAL1 (Product # PA5-29077) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 Irp2 deficiency reduces the ms 2 t 6 A modification in tRNA Lys UUU causing misreading of lysine codons in proinsulin. a Secondary structure and sequence of tRNA Lys UUU with anticodon loop modifications 2-methylthio-N 6 -threonylcarbamoyl adenosine (ms 2 t 6 A37), methoxycarbonylmethyl-2-thiouridine (mcm 5 s 2 U34) and pseudouridine-39 (psi-39). The ms 2 group on ms 2 t 6 A37 is indicated by a dotted circle. Adapted from Vendeix, et al. 68 . b Western blot analysis of Cdkal1 in control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without DFO or FAC for 18 h. beta-Actin is a loading control. Asterisk, nonspecific band. c Western blot analysis of Irp2, Cdkal1, Pcsk1, and CgA in islets isolated from 6-month old WT and Irp2 -/- mice. Gapdh is a loading control for Irp2, Cdkal1 and Pcsk1 and beta-actin is a loading control for CgA ( n = 3 mice per genotype). d - e LC-MS analysis of ms 2 t 6 A ( d ) and t 6 A ( e ) modifications in control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without supplemental FAC for 18 h ( n >= 3 independent biological experiments). f Relative incorporation of 14 C-lysine versus 3 H-leucine in proinsulin immunoprecipitated from control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without supplemental FAC ( n = 3 independent biological experiments). Data in d , e are expressed as means +- s.e.m., unpaired two-tailed Student's t test, * p < 0.05, ** p < 0.01 relative to control INS-1 cells; data in f a
