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AMAb91418

antibody from Atlas Antibodies

Targeting: BCL11B CTIP-2, CTIP2, hRIT1-alpha, ZNF856B

Provider product page for AMAb91418

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [7]

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Validation data

Product number: AMAb91418 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#AMAb91418, RRID:AB_2732085
Product name: Anti-BCL11B
Antibody type: Monoclonal
Antigen: Synthetic peptide
Reactivity: Human, Mouse
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: EIGIQVTPDEDDHLLSPTK
Isotype: IgG
Antibody clone number: CL6426
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

BCL11B protein structure - AMAb91418 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human tonsil and pancreas tissues using AMAb91418 antibody. Corresponding BCL11B RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity, mainly in non - germinal center cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in dermal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of mouse cerebral cortex shows moderate to strong nuclear positivity in neurons, mainly in layers 5-6.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of mouse hippocampus shows strong nuclear positivity in neurons in CA1 pyramidal layer.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of mouse hippocampus shows strong nuclear positivity in neurons in the granular cell layer of dentate gyrus.