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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-119571 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ISL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ISL2 in HeLa whole cell and nuclear extract. Sample was separated with 10% SDS-PAGE, incubated with polyclonal ISL2 antibody (Product # PA5-119571) at a dilution of 1:500, and followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ISL2 in A) 30 μg 293T whole cell lysate/extract; B) 30 μg whole cell lysate/extract of human ISL2-transfected 293T cells. Sample was separated with 10% SDS-PAGE and incubated with polyclonal ISL2 antibody (Product # PA5-119571) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ISL2 in paraffin-embedded mouse spinal cord. Sample was treated for antigen retrieval with Citrate buffer (pH 6.0, 15 min) and incubated with polyclonal ISL2 antibody (Product # PA5-119571) at a dilution of 1:500.
