Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TTLL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, Molt-4, Raji, NIH-3T3.
- Concentration
- 1 mg/mL
Submitted references Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches.
Zuzow N, Ghosh A, Leonard M, Liao J, Yang B, Bennett EJ
Molecular biology of the cell 2018 May 15;29(10):1258-1269
Molecular biology of the cell 2018 May 15;29(10):1258-1269
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TTLL1 Polyclonal Antibody (Product # PA5-27285). Sample (30 µg of whole cell lysate). A: Molt-4. 10% SDS PAGE. TTLL1 Polyclonal Antibody (Product # PA5-27285) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TTLL1 Polyclonal Antibody (Product # PA5-27285). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. TTLL1 Polyclonal Antibody (Product # PA5-27285) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TTLL1 in paraformaldehyde-fixed HeLa cells using a TTLL1 polyclonal antibody (Product # PA5-27285) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5: Ltn1 ubiquitylates a subset of identified interacting proteins in a regulatory manner. (A) 293 Flp-In cells with dox-induced expression of FLAG-HA-tagged wild type or DeltaRING Ltn1 were transfected with HA-ubiquitin and the indicated myc-tagged Ltn1 interacting protein. Whole-cell lysates or anti-HA immune-complexes isolated under native conditions were immunoblotted as indicated. (B) 293 Flp-In cells with dox-induced expression of the indicated FLAG-HA (FH)-tagged proteins were transfected with control scrambled siRNA oligos (siC) or three separate Ltn1-targeting siRNA oligos. Whole-cell extracts were immunoblotted as indicated. (C) 293T cells were transfected with control scrambled siRNA oligos (siC) or three separate Ltn1-targeting siRNA oligos. Whole-cell extracts were immunoblotted as indicated. (D) 293 Flp-In cells with dox-induced expression of FLAG-HA-tagged RSK2, TTLL1, or TYW3 were transfected with control scrambled siRNA oligos (siC) or Ltn1-targeting siRNA oligos. Seventy-two hours after siRNA transfection, cells were either untreated or treated with 100 mug/ml cycloheximide (CHX) for the indicated times. After treatment, cells were harvested and whole-cell extracts were immunoblotted as indicated. (E) 293T cells (left) or HCT116 cells (right) were transfected with control scrambled siRNA oligos (siC) or Ltn1-targeting siRNA oligos. Seventy-two hours after siRNA transfection, cells were either untreated or treated with 100 mug/ml cycloheximide (CHX) for
