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LearnNB100-41398
antibody from Novus Biologicals
Targeting: TSPO
BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-41398 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-41398, RRID:AB_788260
- Product name
- Goat Polyclonal PBR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise one isoform (NP_000705.2) only.
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Synthesis and evaluation of novel potent TSPO PET ligands with 2-phenylpyrazolo[1,5-a]pyrimidin-3-yl acetamide.
Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study.
ERManI (Endoplasmic Reticulum Class I α-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway.
The mitochondrial translocator protein, TSPO, inhibits HIV-1 envelope glycoprotein biosynthesis via the endoplasmic reticulum-associated protein degradation pathway.
The 18-kDa translocator protein (TSPO) disrupts mammary epithelial morphogenesis and promotes breast cancer cell migration.
Peripheral benzodiazepine receptor regulates vascular endothelial activations via suppression of the voltage-dependent anion channel-1.
Translocator protein blockade reduces prostate tumor growth.
Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function.
Hieu Tran V, Park H, Park J, Kwon YD, Kang S, Ho Jung J, Chang KA, Chul Lee B, Lee SY, Kang S, Kim HK
Bioorganic & medicinal chemistry 2019 Sep 15;27(18):4069-4080
Bioorganic & medicinal chemistry 2019 Sep 15;27(18):4069-4080
Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study.
Buck JR, McKinley ET, Fu A, Abel TW, Thompson RC, Chambless L, Watchmaker JM, Harty JP, Cooper MK, Manning HC
PloS one 2015;10(10):e0141659
PloS one 2015;10(10):e0141659
ERManI (Endoplasmic Reticulum Class I α-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway.
Zhou T, Frabutt DA, Moremen KW, Zheng YH
The Journal of biological chemistry 2015 Sep 4;290(36):22184-92
The Journal of biological chemistry 2015 Sep 4;290(36):22184-92
The mitochondrial translocator protein, TSPO, inhibits HIV-1 envelope glycoprotein biosynthesis via the endoplasmic reticulum-associated protein degradation pathway.
Zhou T, Dang Y, Zheng YH
Journal of virology 2014 Mar;88(6):3474-84
Journal of virology 2014 Mar;88(6):3474-84
The 18-kDa translocator protein (TSPO) disrupts mammary epithelial morphogenesis and promotes breast cancer cell migration.
Wu X, Gallo KA
PloS one 2013;8(8):e71258
PloS one 2013;8(8):e71258
Peripheral benzodiazepine receptor regulates vascular endothelial activations via suppression of the voltage-dependent anion channel-1.
Joo HK, Lee YR, Lim SY, Lee EJ, Choi S, Cho EJ, Park MS, Ryoo S, Park JB, Jeon BH
FEBS letters 2012 May 7;586(9):1349-55
FEBS letters 2012 May 7;586(9):1349-55
Translocator protein blockade reduces prostate tumor growth.
Fafalios A, Akhavan A, Parwani AV, Bies RR, McHugh KJ, Pflug BR
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Oct 1;15(19):6177-84
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Oct 1;15(19):6177-84
Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function.
Papadopoulos V, Baraldi M, Guilarte TR, Knudsen TB, Lacapère JJ, Lindemann P, Norenberg MD, Nutt D, Weizman A, Zhang MR, Gavish M
Trends in pharmacological sciences 2006 Aug;27(8):402-9
Trends in pharmacological sciences 2006 Aug;27(8):402-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBR Antibody [NB100-41398] - Western blot analysis of PBR in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NB100-41398 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
