Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-19128 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal HAUS8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against the phosphorylated form of human HAUS8 protein at the pS70 residue. NBP2-19128 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of HAUS8. Reactivity with non-phosphorylated human HAUS8 is minimal by ELISA and western blot.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.22 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HAUS8 [p Ser70] Antibody [NBP2-19128] - Lane 1: HeLa cell extracts of untransfected cells. Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT. Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant. Load: 35 ug per lane. Hice1 pS70 antibody at 0.5 ug/mL for overnight at 4C. Secondary antibody: Dye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band: none.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HAUS8 [p Ser70] Antibody [NBP2-19128] - Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ul per lane. Primary antibody: HICE1 pS70 antibody at 0.75 ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HICE1 pS70. Other band(s): HICE1 pS70 splice variants and isoforms.
